Rhodamin B is a synthetic dye commonly used as a textile dye. Rhodamin B is an additional dye stuff that is prohibited from its use in food products. This substance is designated as dyestuff which is prohibited its use in food product which has been arranged in Regulation of Minister of Health RI. 033 of 2012 on Food Supplementary Material and Regulation of the Minister of Health of the Republic of Indonesia No. 239 / Menkes / Per / V / 1985 on Certain Color Substances declared as hazardous substances. However, the use of Rhodamine B in food is still in the field, such as crackers, sweets,candy, ketchup, sausage and others. Rhodamin B dye although it has been prohibited its use there are still producers who deliberately add Rhodamin B dye to their product. If accumulates in the body Rhodamine B may cause respiratory tract irritation, skin irritation, eye irritation, gastrointestinal irritation, poisoning, liver disorders and can cause cancer.